The Three Lions landed in St Petersburg on Tuesday afternoon en route to their training base in Repino, with the tournament getting under way when hosts Russian Federation face Saudi Arabia on Thursday.
Assuming they survive a favourable group draw that has pitted them against Belgium, Tunisia and Panama, they face a likely knock-out game against Poland or Colombia followed by a possible quarterfinal against Brazil, which would be a repeat of 2002 when England narrowly lost.
"Marcos had a slight knock, although it is not too serious, we will have a look at that before the next training session", commented Southgate, who added England had no further injury worries and that he was "really pleased" with the work the squad had done before flying to Russian Federation.
"Across three weeks, we have been really pleased to get the 23 we picked through all of the sessions".
Rashford shone with a stunning opening goal in England's final warm-up friendly against Costa Rica last week, but England are hopeful he will be fit for their tournament opener against Tunisia on Monday with Southgate describing the injury as a "slight knock".
"I'm confident it won't happen (again) - I won't say 100 percent because you never know in football - but in this team, we wear our heart on our sleeve".
"We are nothing to do with the past", said Southgate in one of his final round of media interviews before they head to their camp in the village of Repino, which is approximately 30 miles from St. Petersburg. We want them to have some ownership.
'Whenever you're in an England shirt you have an opportunity to make history'.
"So to now be managing and leading my country to a World Cup is an extra special moment".
"We did the game last week north v south - obviously the south won - and it's a great togetherness that we've got. We just wanted to get over here and settled to start to prepare for the games ahead", said England captain Harry Kane.
"We're proud to be here, we will work hard, be energetic".
Such potential flash points led to Tottenham defender Danny Rose asking his family to remain behind at home amid fears of racist abuse. "It is about reading the game, I don't want to leave space at the back to get on a counter-attack", he added.